Comparative gene expression study and pathway analysis of the human iris- and the retinal pigment epithelium.

ABSTRACT:

Background

The retinal pigment epithelium (RPE) is a neural monolayer lining the back of the eye. Degeneration of the RPE leads to severe vision loss in, so far incurable, diseases such as age-related macular degeneration and some forms of retinitis pigmentosa. A promising future replacement therapy may be autologous iris epithelial cell transdifferentiation into RPE in vitro and, subsequently, transplantation. In this study we compared the gene expression profiles of the iris epithelium (IE) and the RPE.

Methods

We collected both primary RPE- and IE cells from 5 freshly frozen human donor eyes, using respectively laser dissection microscopy and excision. We performed whole-genome expression profiling using 44k Agilent human microarrays. We investigated the gene expression p

SUBMITTER: Bennis A 

PROVIDER: S-EPMC5565104 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature