Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells.

ABSTRACT: To date, the available surgical and pharmacological treatments for cardiovascular diseases (CVD) are limited and often palliative. At the same time, gene and cell therapies are highly promising alternative approaches for CVD treatment. However, the broad clinical application of gene therapy is greatly limited by the lack of suitable gene delivery systems. The development of appropriate gene delivery vectors can provide a solution to current challenges in cell therapy. In particular, existing drawbacks, such as limited efficiency and low cell retention in the injured organ, could be overcome by appropriate cell engineering (i.e., genetic) prior to transplantation. The presented protocol describes the efficient and safe transient modification of endothelial cells using a polyethyleneimine su

SUBMITTER: Voronina N 

PROVIDER: S-EPMC5565141 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature