Keratin hydrogel carrier system for simultaneous delivery of exogenous growth factors and muscle progenitor cells.

ABSTRACT: Ideal material characteristics for tissue engineering or regenerative medicine approaches to volumetric muscle loss (VML) include the ability to deliver cells, growth factors, and molecules that support tissue formation from a system with a tunable degradation profile. Two different types of human hair-derived keratins were tested as options to fulfill these VML design requirements: (1) oxidatively extracted keratin (keratose) characterized by a lack of covalent crosslinking between cysteine residues, and (2) reductively extracted keratin (kerateine) characterized by disulfide crosslinks. Human skeletal muscle myoblasts cultured on coatings of both types of keratin had increased numbers of multinucleated cells compared to collagen or Matrigel(TM) and adhesion levels greater than collagen.

SUBMITTER: Tomblyn S 

PROVIDER: S-EPMC5565163 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature