Genetic analysis of the Candida albicans biofilm transcription factor network using simple and complex haploinsufficiency.

ABSTRACT: Biofilm formation by Candida albicans is a key aspect of its pathobiology and is regulated by an integrated network of transcription factors (Bcr1, Brg1, Efg1, Ndt80, Rob1, and Tec1). To understand the details of how the transcription factors function together to regulate biofilm formation, we used a systematic genetic interaction approach based on generating all possible double heterozygous mutants of the network genes and quantitatively analyzing the genetic interactions between them. Overall, the network is highly susceptible to genetic perturbation with the six network heterozygous mutants all showing alterations in biofilm formation (haploinsufficiency). In addition, many double heterozygous mutants are as severely affected as homozygous deletions. As a result, the network shows prope

SUBMITTER: Glazier VE 

PROVIDER: S-EPMC5565191 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature