Speckle variance optical coherence tomography of blood flow in the beating mouse embryonic heart.

ABSTRACT: Efficient separation of blood and cardiac wall in the beating embryonic heart is essential and critical for experiment-based computational modelling and analysis of early-stage cardiac biomechanics. Although speckle variance optical coherence tomography (SV-OCT) relying on calculation of intensity variance over consecutively acquired frames is a powerful approach for segmentation of fluid flow from static tissue, application of this method in the beating embryonic heart remains challenging because moving structures generate SV signal indistinguishable from the blood. Here, we demonstrate a modified four-dimensional SV-OCT approach that effectively separates the blood flow from the dynamic heart wall in the beating mouse embryonic heart. The method takes advantage of the periodic motion of

SUBMITTER: Grishina OA 

PROVIDER: S-EPMC5565627 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature