Wnt5a induces catabolic signaling and matrix metalloproteinase production in human articular chondrocytes.

ABSTRACT:

Objective

Aberrant Wnt signaling may contribute to osteoarthritis (OA) but the Wnt family members involved have not been fully identified. The purpose of this study was to investigate the role of Wnt5a as a potential mediator of cartilage destruction in OA.

Design

Immunohistochemistry to detect Wnt5a was performed using normal and OA human articular cartilage. Cultured normal human chondrocytes were treated with fibronectin fragments (FN-f) as a catabolic stimulus or recombinant Wnt5a protein with or without pretreatment using a panel of signaling inhibitors. Expression of Wnt5a, anabolic genes and catabolic genes were determined by quantitative real-time PCR. Production of Wnt5a protein and matrix metalloproteinases (MMPs) as well as activation of signaling proteins were an

SUBMITTER: Huang G 

PROVIDER: S-EPMC5565712 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature