Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry.

ABSTRACT: Quantitative proteomics employing mass spectrometry is an indispensable tool in life science research. Targeted proteomics has emerged as a powerful approach for reproducible quantification but is limited in the number of proteins quantified. SWATH-mass spectrometry consists of data-independent acquisition and a targeted data analysis strategy that aims to maintain the favorable quantitative characteristics (accuracy, sensitivity, and selectivity) of targeted proteomics at large scale. While previous SWATH-mass spectrometry studies have shown high intra-lab reproducibility, this has not been evaluated between labs. In this multi-laboratory evaluation study including 11 sites worldwide, we demonstrate that using SWATH-mass spectrometry data acquisition we can consistently detect and reprodu

SUBMITTER: Collins BC 

PROVIDER: S-EPMC5566333 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature