Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection.

ABSTRACT: In this study, we fabricated a novel electrochemical biosensing platform on the basis of target-triggered proximity hybridization-mediated isothermal exponential amplification reaction (EXPAR) for ultrasensitive protein analysis. Through rational design, the aptamers for protein recognition were integrated within two DNA probes. Via proximity hybridization principle, the affinity protein-binding event was converted into DNA assembly process. The recognition of protein by aptamers can trigger the strand displacement through the increase of the local concentrations of the involved probes. As a consequence, the output DNA was displaced, which can hybridize with the duplex probes immobilized on the electrode surface subsequently, leading to the initiation of the EXPAR as well as the cleavage o

SUBMITTER: Yu Y 

PROVIDER: S-EPMC5566414 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature