MatCol: a tool to measure fluorescence signal colocalisation in biological systems.

ABSTRACT: Protein colocalisation is often studied using pixel intensity-based coefficients such as Pearson, Manders, Li or Costes. However, these methods cannot be used to study object-based colocalisations in biological systems. Therefore, a novel method is required to automatically identify regions of fluorescent signal in two channels, identify the co-located parts of these regions, and calculate the statistical significance of the colocalisation. We have developed MatCol to address these needs. MatCol can be used to visualise protein and/or DNA colocalisations and fine tune user-defined parameters for the colocalisation analysis, including the application of median or Wiener filtering to improve the signal to noise ratio. Command-line execution allows batch processing of multiple images. Users c

SUBMITTER: Khushi M 

PROVIDER: S-EPMC5566543 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature