A complex of Neuroplastin and Plasma Membrane Ca²⁺ ATPase controls T cell activation.

ABSTRACT: The outcome of T cell activation is determined by mechanisms that balance Ca²⁺ influx and clearance. Here we report that murine CD4 T cells lacking Neuroplastin (Nptn ^-/-), an immunoglobulin superfamily protein, display elevated cytosolic Ca²⁺ and impaired post-stimulation Ca²⁺ clearance, along with increased nuclear levels of NFAT transcription factor and enhanced T cell receptor-induced cytokine production. On the molecular level, we identified plasma membrane Ca²⁺ ATPases (PMCAs) as the main interaction partners of Neuroplastin. PMCA levels were reduced by over 70% in Nptn ^-/- T cells, suggesting an explanation for altered Ca²⁺ handling. Supporting this, Ca²⁺ extrusion was impaired while Ca²⁺

SUBMITTER: Korthals M 

PROVIDER: S-EPMC5566957 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature