In vivo Assembly in Escherichia coli of Transformation Vectors for Plastid Genome Engineering.

ABSTRACT: Plastid transformation for the expression of recombinant proteins and entire metabolic pathways has become a promising tool for plant biotechnology. However, large-scale application of this technology has been hindered by some technical bottlenecks, including lack of routine transformation protocols for agronomically important crop plants like rice or maize. Currently, there are no standard or commercial plastid transformation vectors available for the scientific community. Construction of a plastid transformation vector usually requires tedious and time-consuming cloning steps. In this study, we describe the adoption of an in vivo Escherichia coli cloning (iVEC) technology to quickly assemble a plastid transformation vector. The method enables simple and seamless build-up of a comp

SUBMITTER: Wu Y 

PROVIDER: S-EPMC5566966 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature