Restriction and modification of deoxyarchaeosine (dG⁺)-containing phage 9 g DNA.

ABSTRACT: E. coli phage 9 g contains the modified base deoxyarchaeosine (dG⁺) in its genome. The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to synthesize and incorporate dG⁺. Here we report phage 9 g DNA sensitivity to >200 Type II restriction endonucleases (REases). Among the REases tested approximately 29% generated complete or partial digestions, while the remaining 71% displayed resistance to restriction. Phage 9 g restriction fragments can be degraded by DNA exonucleases or ligated by T3 and T4 DNA ligases. In addition, we examined a number of cytosine and adenine methyltransferases to generate double base modifications. M.AluI, M.CviPI, M.HhaI, and M.EcoGII were able to introduce ^5mC or ^N6mA into 9 g DNA as confi

SUBMITTER: Tsai R 

PROVIDER: S-EPMC5567051 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature