Survey of methanotrophic diversity in various ecosystems by degenerate methane monooxygenase gene primers.

ABSTRACT: Methane is the second most important greenhouse gas contributing to about 20% of global warming. Its mitigation is conducted by methane oxidizing bacteria that act as a biofilter using methane as their energy and carbon source. Since their first discovery in 1906, methanotrophs have been studied using a complementary array of methods. One of the most used molecular methods involves PCR amplification of the functional gene marker for the diagnostic of copper and iron containing particulate methane monooxygenase. To investigate the diversity of methanotrophs and to extend their possible molecular detection, we designed a new set of degenerate methane monooxygenase primers to target an 850 nucleotide long sequence stretch from pmoC to pmoA. The primers were based on all available full genomic

SUBMITTER: Ghashghavi M 

PROVIDER: S-EPMC5567572 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature