Amyloid β concentrations and stable isotope labeling kinetics of human plasma specific to central nervous system amyloidosis.

ABSTRACT:

Introduction

Cerebrospinal fluid analysis and other measurements of amyloidosis, such as amyloid-binding positron emission tomography studies, are limited by cost and availability. There is a need for a more practical amyloid β (Aβ) biomarker for central nervous system amyloid deposition.

Methods

We adapted our previously reported stable isotope labeling kinetics protocol to analyze the turnover kinetics and concentrations of Aβ38, Aβ40, and Aβ42 in human plasma.

Results

Aβ isoforms have a half-life of approximately 3 hours in plasma. Aβ38 demonstrated faster turnover kinetics compared with Aβ40 and Aβ42. Faster fractional turnover of Aβ42 relative to Aβ40 and lower Aβ42 and Aβ42/Aβ40 concentrations in amyloid-positive participants were observed.

Discussion

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SUBMITTER: Ovod V 

PROVIDER: S-EPMC5567785 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature