Variants of sequence family B Thermococcus kodakaraensis DNA polymerase with increased mismatch extension selectivity.

ABSTRACT: Fidelity and selectivity of DNA polymerases are critical determinants for the biology of life, as well as important tools for biotechnological applications. DNA polymerases catalyze the formation of DNA strands by adding deoxynucleotides to a primer, which is complementarily bound to a template. To ensure the integrity of the genome, DNA polymerases select the correct nucleotide and further extend the nascent DNA strand. Thus, DNA polymerase fidelity is pivotal for ensuring that cells can replicate their genome with minimal error. DNA polymerases are, however, further optimized for more specific biotechnological or diagnostic applications. Here we report on the semi-rational design of mutant libraries derived by saturation mutagenesis at single sites of a 3'-5'-exonuclease deficient varian

SUBMITTER: Huber C 

PROVIDER: S-EPMC5568139 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature