Identification and analysis of alternative splicing events in Phaseolus vulgaris and Glycine max.

ABSTRACT:

Background

The vast diversification of proteins in eukaryotic cells has been related with multiple transcript isoforms from a single gene that result in alternative splicing (AS) of primary transcripts. Analysis of RNA sequencing data from expressed sequence tags and next generation RNA sequencing has been crucial for AS identification and genome-wide AS studies. For the identification of AS events from the related legume species Phaseolus vulgaris and Glycine max, 157 and 88 publicly available RNA-seq libraries, respectively, were analyzed.

Results

We identified 85,570 AS events from P. vulgaris in 72% of expressed genes and 134,316 AS events in 70% of expressed genes from G. max. These were categorized in seven AS event types with intron retention being the most abundant f

SUBMITTER: Iniguez LP 

PROVIDER: S-EPMC5568362 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature