Cristae architecture is determined by an interplay of the MICOS complex and the F₁F_O ATP synthase via Mic27 and Mic10.

ABSTRACT: The inner boundary and the cristae membrane are connected by pore-like structures termed crista junctions (CJs). The MICOS complex is required for CJ formation and enriched at CJs. Here, we address the roles of the MICOS subunits Mic27 and Mic10. We observe a positive genetic interaction between Mic27 and Mic60 and deletion of Mic27 results in impaired formation of CJs and altered cristae membrane curvature. Mic27 acts in an antagonistic manner to Mic60 as it promotes oligomerization of the F₁F_O-ATP synthase and partially restores CJ formation in cells lacking Mic60. Mic10 impairs oligomerization of the F₁F_O-ATP synthase similar to Mic60. Applying complexome profiling, we observed that deletion of Mic27 destabilizes the MICOS complex but does not

SUBMITTER: Eydt K 

PROVIDER: S-EPMC5568431 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature