Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells.

ABSTRACT: Delivery of various forms of recombinant Theileria parva sporozoite antigen (p67) has been shown to elicit antibody responses in cattle capable of providing protection against East Coast fever, the clinical disease caused by T. parva. Previous formulations of full-length and shorter recombinant versions of p67 derived from bacteria, insect, and mammalian cell systems are expressed in non-native and highly unstable forms. The stable expression of full-length recombinant p67 in mammalian cells has never been described and has remained especially elusive. In this study, p67 was expressed in human-derived cells as a full-length, membrane-linked protein and as a secreted form by omission of the putative transmembrane domain. The recombinant protein expressed in this system yielded primarily two

SUBMITTER: Tebaldi G 

PROVIDER: S-EPMC5568440 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature