Association between ROS production, swelling and the respirasome integrity in cardiac mitochondria.

ABSTRACT: Although mitochondrial Ca²⁺ overload and ROS production play a critical role in mitochondria-mediated cell death, a cause-effect relationship between them remains elusive. This study elucidated the crosstalk between mitochondrial swelling, ROS production, and electron transfer chain (ETC) supercomplexes in rat heart mitochondria in response to Ca²⁺ and tert-butyl hydroperoxide (TBH), a lipid-soluble organic peroxide. Results showed that ROS production induced by TBH was significantly increased in the presence of Ca²⁺ in a dose-dependent manner. TBH markedly inhibited the state 3 respiration rate with no effect on the mitochondrial swelling. Ca²⁺ exerted a slight effect on mitochondrial respiration that was greatly aggravated by TBH. Analysis of s

SUBMITTER: Jang S 

PROVIDER: S-EPMC5568452 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature