GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.

ABSTRACT: The separation of germline from somatic lineages is fundamental to reproduction and species preservation. Here, we show that Drosophila Germ cell-less (GCL) is a critical component in this process by acting as a switch that turns off a somatic lineage pathway. GCL, a conserved BTB (Broad-complex, Tramtrack, and Bric-a-brac) protein, is a substrate-specific adaptor for Cullin3-RING ubiquitin ligase complex (CRL3^GCL). We show that CRL3^GCL promotes PGC fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate. This mode of RTK degradation does not depend upon receptor activation but is prompted by release of GCL from the nuclear envelope during mitosis. The cell-cycle-dependent change in GCL localization provides

SUBMITTER: Pae J 

PROVIDER: S-EPMC5568677 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature