A DNA dot hybridization model for molecular diagnosis of parasitic keratitis.

ABSTRACT:

Purpose

Developing a DNA dot hybridization model for diagnosing parasitic keratitis.

Methods

Newly designed oligonucleotide probes for detecting Acanthamoeba and microsporidia were tested with target reference strains of Acanthamoeba (n = 20) and microsporidia (n = 3), and non-target microorganisms, including bacteria (n = 20) and fungi (n = 20). These probes, which had passed the preliminary tests, were then assembled as a parasite dot hybridization (PDH) model for assessing 33 clinical samples from patients with clinically suspected Acanthamoeba and microsporidia keratitis, including eight positives for Acanthamoeba, 13 positives for microsporidia, and 12 negatives for both pathogens.

Results

Two probes for detecting Acanthamoeba a

SUBMITTER: Huang FC 

PROVIDER: S-EPMC5568909 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature