RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes.

ABSTRACT: Synthetic biology and metabolic engineering seek to re-engineer microbes into "living foundries" for the production of high value chemicals. Through a "design-build-test" cycle paradigm, massive libraries of genetically engineered microbes can be constructed and tested for metabolite overproduction and secretion. However, library generation capacity outpaces the rate of high-throughput testing and screening. Well plate assays are flexible but with limited throughput, whereas droplet microfluidic techniques are ultrahigh-throughput but require a custom assay for each target. Here we present RNA-aptamers-in-droplets (RAPID), a method that greatly expands the generality of ultrahigh-throughput microfluidic screening. Using aptamers, we transduce extracellular product titer into fluorescence,

SUBMITTER: Abatemarco J 

PROVIDER: S-EPMC5569033 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature