Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors.

ABSTRACT: PDX1⁺/NKX6-1⁺ pancreatic progenitors (PPs) give rise to endocrine cells both in vitro and in vivo. This cell population can be successfully differentiated from human pluripotent stem cells (hPSCs) and hold the potential to generate an unlimited supply of β cells for diabetes treatment. However, the efficiency of PP generation in vitro is highly variable, negatively impacting reproducibility and validation of in vitro and in vivo studies, and consequently, translation to the clinic. Here, we report the use of a proteomics approach to phenotypically characterize hPSC-derived PPs and distinguish these cells from non-PP populations during differentiation. Our analysis identifies the pancreatic secretory granule membrane major glycoprotein 2 (GP2) as a PP-specific cell sur

SUBMITTER: Cogger KF 

PROVIDER: S-EPMC5569081 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature