Development of a versatile and conventional technique for gene disruption in filamentous fungi based on CRISPR-Cas9 technology.

ABSTRACT: Filamentous fungi represent an invaluable source of pharmaceutically active compounds. The development of versatile methods to genetically manipulate filamentous fungi is of great value for improving the low yields of bioactive metabolites and expanding chemical diversity. The CRISPR-Cas9-based system has become a common platform for genome editing in a variety of organisms. However, recent application of this technology in filamentous fungi is limited to model strains, a versatile method for efficient gene disruption in different fungi is lacking. Here, we investigated the utility of the CRISPR-Cas9 system in a less-studied fungus Nodulisporium sp. (No. 65-12-7-1), and we have developed an efficient CRISPR-Cas9-based gene disruption strategy by simultaneous transformation of in vitro tran

SUBMITTER: Zheng YM 

PROVIDER: S-EPMC5569088 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature