CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools.

ABSTRACT: Robust and cost-effective genome editing in a diverse array of cells and model organisms is now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas system. The commonly used Cas9 of Streptococcus pyogenes shows high levels of activity but, depending on the application, has been associated with some shortcomings. Firstly, the enzyme has been shown to cause mutagenesis at genomic sequences resembling the target sequence. Secondly, the stringent requirement for a specific motif adjacent to the selected target site can limit the target range of this enzyme. Lastly, the physical size of Cas9 challenges the efficient delivery of genomic engineering tools based on this enzyme as viral particles for potential therapeutic applications. Related and parallel strategies

SUBMITTER: Cebrian-Serrano A 

PROVIDER: S-EPMC5569134 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature