Effector and regulatory dendritic cells display distinct patterns of miRNA expression.

ABSTRACT:

Introduction

MicroRNAs (miRNAs) contribute to the regulation of dendritic cell (DC) polarization, thereby influencing the balance of adaptive immune responses. Herein, we studied the expression of miRNAs in polarized DCs and analyzed whether expression of these miRNAs could be associated with allergic rhinitis and allergen immunotherapy (AIT) outcome.

Method

Using specific culture conditions, we differentiated immature human monocyte-derived DCs into DC1, DC2, and DCreg subsets (supporting the differentiation of T_H 1, T_H 2 or regulatory T cells, respectively). Profiling of miRNA expression was performed in these DC subpopulations using microarrays. Levels of miRNAs specific for polarized DCs were then evaluated in a cohort of 58 patients with allergic r

SUBMITTER: Lombardi V 

PROVIDER: S-EPMC5569363 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature