Droplet Barcode Sequencing for targeted linked-read haplotyping of single DNA molecules.

ABSTRACT: Data produced with short-read sequencing technologies result in ambiguous haplotyping and a limited capacity to investigate the full repertoire of biologically relevant forms of genetic variation. The notion of haplotype-resolved sequencing data has recently gained traction to reduce this unwanted ambiguity and enable exploration of other forms of genetic variation; beyond studies of just nucleotide polymorphisms, such as compound heterozygosity and structural variations. Here we describe Droplet Barcode Sequencing, a novel approach for creating linked-read sequencing libraries by uniquely barcoding the information within single DNA molecules in emulsion droplets, without the aid of specialty reagents or microfluidic devices. Barcode generation and template amplification is performed simul

SUBMITTER: Redin D 

PROVIDER: S-EPMC5569991 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature