Interlinked DNA nano-circles for measuring topoisomerase II activity at the level of single decatenation events.

ABSTRACT: DNA nano-structures present appealing new means for monitoring different molecules. Here, we demonstrate the assembly and utilization of a surface-attached double-stranded DNA catenane composed of two intact interlinked DNA nano-circles for specific and sensitive measurements of the life essential topoisomerase II (Topo II) enzyme activity. Topo II activity was detected via the numeric release of DNA nano-circles, which were visualized at the single-molecule level in a fluorescence microscope upon isothermal amplification and fluorescence labeling. The transition of each enzymatic reaction to a micrometer sized labeled product enabled quantitative detection of Topo II activity at the single decatenation event level rendering activity measurements in extracts from as few as five cells possi

SUBMITTER: Kristoffersen EL 

PROVIDER: S-EPMC5570003 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature