EIF1 modulates the recognition of suboptimal translation initiation sites and steers gene expression via uORFs.

ABSTRACT: Alternative translation initiation mechanisms such as leaky scanning and reinitiation potentiate the polycistronic nature of human transcripts. By allowing for reprogrammed translation, these mechanisms can mediate biological responses to stimuli. We combined proteomics with ribosome profiling and mRNA sequencing to identify the biological targets of translation control triggered by the eukaryotic translation initiation factor 1 (eIF1), a protein implicated in the stringency of start codon selection. We quantified expression changes of over 4000 proteins and 10 000 actively translated transcripts, leading to the identification of 245 transcripts undergoing translational control mediated by upstream open reading frames (uORFs) upon eIF1 deprivation. Here, the stringency of start codon selec

SUBMITTER: Fijalkowska D 

PROVIDER: S-EPMC5570006 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature