Quantifying the Number of Independent Organelle DNA Insertions in Genome Evolution and Human Health.

ABSTRACT: Fragments of organelle genomes are often found as insertions in nuclear DNA. These fragments of mitochondrial DNA (numts) and plastid DNA (nupts) are ubiquitous components of eukaryotic genomes. They are, however, often edited out during the genome assembly process, leading to systematic underestimation of their frequency. Numts and nupts, once inserted, can become further fragmented through subsequent insertion of mobile elements or other recombinational events that disrupt the continuity of the inserted sequence relative to the genuine organelle DNA copy. Because numts and nupts are typically identified through sequence comparison tools such as BLAST, disruption of insertions into smaller fragments can lead to systematic overestimation of numt and nupt frequencies. Accurate identificatio

SUBMITTER: Hazkani-Covo E 

PROVIDER: S-EPMC5570036 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature