Molecular envelope and atomic model of an anti-terminated glyQS T-box regulator in complex with tRNAGly.

ABSTRACT: A T-box regulator or riboswitch actively monitors the levels of charged/uncharged tRNA and participates in amino acid homeostasis by regulating genes involved in their utilization or biosynthesis. It has an aptamer domain for cognate tRNA recognition and an expression platform to sense the charge state and modulate gene expression. These two conserved domains are connected by a variable linker that harbors additional secondary structural elements, such as Stem III. The structural basis for specific tRNA binding is known, but the structural basis for charge sensing and the role of other elements remains elusive. To gain new structural insights on the T-box mechanism, a molecular envelope was calculated from small angle X-ray scattering data for the Bacillus subtilis glyQS T-box riboswitch i

SUBMITTER: Chetnani B 

PROVIDER: S-EPMC5570125 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature