Glioblastoma stem cell differentiation into endothelial cells evidenced through live-cell imaging.

ABSTRACT:

Background

Glioblastoma cell-initiated vascularization is an alternative angiogenesis called vasculogenic mimicry. However, current knowledge on the mechanism of de novo vessel formation from glioblastoma stem cells (GSCs) is limited.

Methods

Sixty-four glioblastoma samples from patients and 10 fluorescent glioma xenograft samples were examined by immunofluorescence staining for endothelial marker (CD34 and CD31) and glial cell marker (glial fibrillary acidic protein [GFAP]) expression. GSCs were then isolated from human glioblastoma tissue and CD133+/Sox2+ red fluorescent protein-containing (RFP)-GSC-1 cells were established. The ability of these cells to form vascular structures was examined by live-cell imaging of 3D cultures.

Results

CD34-GFAP or CD31-GFAP coexpre

SUBMITTER: Mei X 

PROVIDER: S-EPMC5570159 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature