Multidimensional pooled shRNA screens in human THP-1 cells identify candidate modulators of macrophage polarization.

ABSTRACT: Macrophages are key cell types of the innate immune system regulating host defense, inflammation, tissue homeostasis and cancer. Within this functional spectrum diverse and often opposing phenotypes are displayed which are dictated by environmental clues and depend on highly plastic transcriptional programs. Among these the 'classical' (M1) and 'alternative' (M2) macrophage polarization phenotypes are the best characterized. Understanding macrophage polarization in humans may reveal novel therapeutic intervention possibilities for chronic inflammation, wound healing and cancer. Systematic loss of function screening in human primary macrophages is limited due to lack of robust gene delivery methods and limited sample availability. To overcome these hurdles we developed cell-autonomous assay

SUBMITTER: Surdziel E 

PROVIDER: S-EPMC5570424 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature