A 'synthetic-sickness' screen for senescence re-engagement targets in mutant cancer backgrounds.

ABSTRACT: Senescence is a universal barrier to immortalisation and tumorigenesis. As such, interest in the use of senescence-induction in a therapeutic context has been gaining momentum in the past few years; however, senescence and immortalisation remain underserved areas for drug discovery owing to a lack of robust senescence inducing agents and an incomplete understanding of the signalling events underlying this complex process. In order to address this issue we undertook a large-scale morphological siRNA screen for inducers of senescence phenotypes in the human melanoma cell line A375P. Following rescreen and validation in a second cancer cell line, HCT116 colorectal carcinoma, a panel of 16 of the most robust hits were selected for further validation based on significance and the potential to b

SUBMITTER: Cairney CJ 

PROVIDER: S-EPMC5570495 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature