Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA.

ABSTRACT: In response to DNA damage during S phase, cells slow DNA replication. This slowing is orchestrated by the intra-S checkpoint and involves inhibition of origin firing and reduction of replication fork speed. Slowing of replication allows for tolerance of DNA damage and suppresses genomic instability. Although the mechanisms of origin inhibition by the intra-S checkpoint are understood, major questions remain about how the checkpoint regulates replication forks: Does the checkpoint regulate the rate of fork progression? Does the checkpoint affect all forks, or only those encountering damage? Does the checkpoint facilitate the replication of polymerase-blocking lesions? To address these questions, we have analyzed the checkpoint in the fission yeast Schizosaccharomyces pombe using a single-mo

SUBMITTER: Iyer DR 

PROVIDER: S-EPMC5570505 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature