Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry.

ABSTRACT: Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially express

SUBMITTER: Rolandsson Enes S 

PROVIDER: S-EPMC5570998 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature