Identification and Mode of Action of a Plant Natural Product Targeting Human Fungal Pathogens.

ABSTRACT: Candida albicans is a major cause of fungal diseases in humans, and its resistance to available drugs is of concern. In an attempt to identify novel antifungal agents, we initiated a small-scale screening of a library of 199 natural plant compounds (i.e., natural products [NPs]). In vitro susceptibility profiling experiments identified 33 NPs with activity against C. albicans (MIC₅₀s ≤ 32 μg/ml). Among the selected NPs, the sterol alkaloid tomatidine was further investigated. Tomatidine originates from the tomato (Solanum lycopersicum) and exhibited high levels of fungistatic activity against Candida species (MIC₅₀s ≤ 1 μg/ml) but no cytotoxicity against mammalian cells. Genome-wide transcriptional analysis of tomatidine-treated C

SUBMITTER: Dorsaz S 

PROVIDER: S-EPMC5571344 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature