The pathogenicity of T cell epitopes on human Goodpasture antigen and its critical amino acid motif.

ABSTRACT: Goodpasture antigen, the non-collagenous domain of α3 chain of type IV collagen [α3(IV)NC1], is the target antigen of anti-glomerular basement membrane (GBM) antibodies. The pathogenicity of T cell epitopes is not elucidated clearly. In this study, we aim to define the nephritogenic T cell epitopes and its critical amino acid residues. Twenty-four overlapping linear peptides were synthesized covering the whole sequence of human α3(IV)NC1. Wistar-Kyoto rats were immunized with linear peptides, and experimental autoimmune glomerulonephritis was evaluated. Critical amino acid was identified by the loss of nephritogenic function after each amino acid substitution by alanine. Of the 24 peptides, P14 (α3_127-148 ) could induce 90.5% (19/21) of WKY rats developing anti-GBM glomeruloneph

SUBMITTER: Hu SY 

PROVIDER: S-EPMC5571546 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature