Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida.

ABSTRACT:

Background

Sedentary endoparasitic cyst nematodes form a feeding structure in plant roots, called a syncytium. Syncytium formation involves extensive transcriptional modifications, which leads to cell modifications such as increased cytoplasmic streaming, enlarged nuclei, increased numbers of organelles, and replacement of a central vacuole by many small vacuoles. When whole root RNA is isolated and analyzed, transcript changes manifested in the infected plant cells are overshadowed by gene expression from cells of the entire root system. Use of microaspiration allows isolation of the content of nematode infected cells from a heterogeneous cell population. However, one challenge with this method is identifying the nematode infected cells under the microscope at early stages of infe

SUBMITTER: Kooliyottil R 

PROVIDER: S-EPMC5571573 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature