MicroRNA expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli in vitro.

ABSTRACT:

Background

Coliform mastitis is a symptom of postpartum dysgalactia syndrome (PDS), a multifactorial infectious disease of sows. Our previous study showed gene expression profile change after bacterial challenge of porcine mammary epithelial cells (PMECs). These mRNA expression changes may be regulated through microRNAs (miRNAs) which play critical roles in biological processes. Therefore, miRNA expression profile was investigated in PMECs.

Results

PMECs were isolated from three lactating sows and challenged with heat-inactivated potential mastitis-causing pathogen Escherichia coli (E. coli) for 3 h and 24 h, in vitro. At 3 h post-challenge with E. coli, target gene prediction identified a critical role of miRNAs in regulation of host immune responses and homeostasis of PMEC

SUBMITTER: Jaeger A 

PROVIDER: S-EPMC5571640 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature