Validation of Geno-Sen's Scrub Typhus Real Time Polymerase Chain Reaction Kit by its Comparison with a Serological ELISA Test.

ABSTRACT:

Background

In the recent past, scrub typhus (ST) has been reported from different parts of India, based on Weil-Felix/enzyme-linked immunosorbent assay (ELISA)/indirect immunofluorescence assay (IFA). Molecular tests are applied only by a few researchers.

Aims

Evaluation of a new commercial real time polymerase chain reaction (PCR) kit for molecular diagnosis of ST by comparing it with the commonly used IgM ELISA is our aim.

Settings and design

ST has been reported all over India including Puducherry and surrounding Tamil Nadu and identified as endemic for ST. This study was designed to correlate antibody detection by IgM ELISA and Orientia tsutsugamushi DNA in real time PCR.

Materials and methods

ST IgM ELISA (InBios Inc., USA) was carried out for 170 c

SUBMITTER: Anitharaj V 

PROVIDER: S-EPMC5572194 | biostudies-literature | 2017 Jul-Sep

REPOSITORIES: biostudies-literature