Thermodynamics and kinetics of RNA tertiary structure formation in the junctionless hairpin ribozyme.

ABSTRACT: The hairpin ribozyme consists of two RNA internal loops that interact to form the catalytically active structure. This docking transition is a rare example of intermolecular formation of RNA tertiary structure without coupling to helix annealing. We have used temperature-dependent surface plasmon resonance (SPR) to characterize the thermodynamics and kinetics of RNA tertiary structure formation for the junctionless form of the ribozyme, in which loops A and B reside on separate molecules. We find docking to be strongly enthalpy-driven and to be accompanied by substantial activation barriers for association and dissociation, consistent with the structural reorganization of both internal loops upon complex formation. Comparisons with the parallel analysis of a ribozyme variant carrying a 2'-

SUBMITTER: White NA 

PROVIDER: S-EPMC5572644 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature