Dataset Information

The Role of Periostin in the Occurrence and Progression of Eosinophilic Chronic Sinusitis with Nasal Polyps.

ABSTRACT: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a highly heterogeneous disease with different host defence responses. However, whether periostin and vascular endothelial growth factor (VEGF) are similarly impaired in patients with eosinophilic CRSwNP (ENP) and those with non-eosinophilic CRSwNP (nENP) remains unclear. We sought to evaluate the expression and possible modulation of periostin and VEGF, regulated on activation normal T expressed and secreted (RANTES) and eotaxin-2 in the polyp tissues from 30 patients with ENP and from 36 patients with nENP and in middle turbinate tissues from 12 control subjects. We found that ENP tissues exhibited a significantly increased expression of periostin and VEGF compared with tissues from patients with nENP and control subjects (P < 0.05, respectively). Accordingly, the expression of VEGF, RANTES, and eotaxin-2 in ENP fibroblasts was significantly up-regulated after stimulation with up-regulated periostin in vitro, but the expression of VEGF and RANTES was significantly inhibited by stimulation with down-regulated periostin. Our findings suggest that periostin might play an important role in the occurrence and progression of ENP and might be a potential therapeutic target.

SUBMITTER: Xu M

PROVIDER: S-EPMC5572682 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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The Role of Periostin in the Occurrence and Progression of Eosinophilic Chronic Sinusitis with Nasal Polyps.

Xu Ming M Chen Daishi D Zhou Haojie H Zhang Weiwei W Xu Jun J Chen Lei L

Scientific reports 20170825 1

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a highly heterogeneous disease with different host defence responses. However, whether periostin and vascular endothelial growth factor (VEGF) are similarly impaired in patients with eosinophilic CRSwNP (ENP) and those with non-eosinophilic CRSwNP (nENP) remains unclear. We sought to evaluate the expression and possible modulation of periostin and VEGF, regulated on activation normal T expressed and secreted (RANTES) and eotaxin-2 in the polyp ...[more]

PMID: 28842563

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Project description:BackgroundRecent literature has indicated the feasibility of microarray analysis in the characterization of chronic sinusitis. We hypothesized that previously unexplored inflammatory mechanisms would be involved in the pathophysiology of noneosinophilic chronic rhinosinusitis with nasal polyps (NE-CRSwNP) and that this technology could be used to identify the gene expression of these novel and previously known mediators.MethodsPatients with CRSwNP failing medical therapy were prospectively enrolled and NP tissue was removed at time of surgery. NE-CRSwNP was diagnosed based on clinical parameters including absence of allergic disease and confirmed with histopathology showing lack of eosinophilic infiltration. Messenger RNA (mRNA) transcripts extracted from study and control patients were then subjected to microarray analysis using Affymatrix based chips. Validation of findings was then confirmed via quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsMicroarray analysis revealed activation of pathways involved in antigen presentation, cellular movement, hematopoiesis, carcinogenesis, apoptosis, and cell signaling. Previously unexplored genes of interest were identified and their differential regulation was validated via qRT-PCR. Our data showed up-regulation of innate inflammation genes (IL-6, IL-8, and monocyte chemoattractant protein 1), hypoxia-induced inflammation 1alpha, and fibrosis (tenascin) and lack of up-regulation of genes associated with allergic, eosinophilic inflammation (IL-4 and IL-13). Additionally, the genes for CXCL1 and autocrine motility factor receptor were novelly identified to be up-regulated.ConclusionThis study explores the utility of gene microarray technology in identifying unexplored targets of immune dysregulation in NE-CRSwNP. Furthermore, the data characterize the immunologic profile of NE-CRSwNP as it differs from other forms of CRSwNP, in particular, those known to be associated with eosinophilic inflammation.

Project description:BackgroundEpidemiological investigations provide considerable evidence supporting the coexistence of upper airway ailments with lower airway disorders, but the association between common nasal diseases, such as allergic rhinitis, chronic sinusitis, nasal polyps, and chronic respiratory conditions require further exploration.MethodsIn this study, a two-sample mendelian randomization was employed to explore the potential association between allergic rhinitis, nasal polyps, and chronic sinusitis with various chronic respiratory diseases. For the primary analysis, summary statistics related to chronic respiratory diseases were obtained from the UK Biobank of European ancestry. To externally validate the results, summary statistics related to chronic respiratory diseases were sourced from the FinnGen R10 database. The analysis incorporated various methodologies, including the inverse variance weighted method, the MR Egger method, and the weighted median method. Sensitivity analysis encompassed Cochran 's Q test, MR-Egger intercept tests, leave-one-out analyses, and the construction of funnel plots.ResultsAllergic rhinitis was significantly associated with asthma (UKB database, OR 1.082, 95% CI 1.072-1.0924, P < 0.001; FinnGen database, OR 1.382, 95% CI 1.305-1.462, P < 0.001), COPD (UKB database, OR 1.003, 95% CI 1.001-1.006, P = 0.020; FinnGen database, OR 1.102, 95% CI 1.037-1.172, P = 0.002), ILD (UKB database, OR 1.013, 95% CI 1.010-1.017, P < 0.001; FinnGen database, OR 1.152, 95% CI 1.035-1.283, P = 0.010). Nasal polyps were potentially related to the increased risks of COPD (UKB database, OR 1.003, 95% CI 1.001-1.004, P < 0.001; FinnGen database, OR 1.092, 95% CI 1.050-1.136, P < 0.001) and bronchiectasis (UKB database, OR 1.000, 95% CI 1.000-1.001, P = 0.036; FinnGen database, OR 1.109, 95% CI 1.022-1.203, P = 0.013).ConclusionsThis study indicates a potential relationship between allergic rhinitis and respiratory conditions such as asthma, chronic obstructive pulmonary disease (COPD), and interstitial lung disease (ILD). Additionally, the presence of nasal polyps appears to be correlated with an increased prevalence of COPD and bronchiectasis.

Dataset Information

The Role of Periostin in the Occurrence and Progression of Eosinophilic Chronic Sinusitis with Nasal Polyps.

Publications

The Role of Periostin in the Occurrence and Progression of Eosinophilic Chronic Sinusitis with Nasal Polyps.

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