Kollector: transcript-informed, targeted de novo assembly of gene loci.

ABSTRACT:

Motivation

Despite considerable advancements in sequencing and computing technologies, de novo assembly of whole eukaryotic genomes is still a time-consuming task that requires a significant amount of computational resources and expertise. A targeted assembly approach to perform local assembly of sequences of interest remains a valuable option for some applications. This is especially true for gene-centric assemblies, whose resulting sequence can be readily utilized for more focused biological research. Here we describe Kollector, an alignment-free targeted assembly pipeline that uses thousands of transcript sequences concurrently to inform the localized assembly of corresponding gene loci. Kollector robustly reconstructs introns and novel sequences within these loci, and scales we

SUBMITTER: Kucuk E 

PROVIDER: S-EPMC5572715 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature