Therapeutic Antibodies to Ganglioside GD2 Evolved from Highly Selective Germline Antibodies.

ABSTRACT: Antibodies play a crucial role in host defense and are indispensable research tools, diagnostics, and therapeutics. Antibody generation involves binding of genomically encoded germline antibodies followed by somatic hypermutation and in vivo selection to obtain antibodies with high affinity and selectivity. Understanding this process is critical for developing monoclonal antibodies, designing effective vaccines, and understanding autoantibody formation. Prior studies have found that antibodies to haptens, peptides, and proteins evolve from polyspecific germline antibodies. The immunological evolution of antibodies to mammalian glycans has not been studied. Using glycan microarrays, protein microarrays, cell binding studies, and molecular modeling, we demonstrate that therapeutic antibodies

SUBMITTER: Sterner E 

PROVIDER: S-EPMC5572838 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature