Contrasting effects of phosphatidylinositol 4,5-bisphosphate on cloned TMEM16A and TMEM16B channels.

ABSTRACT:

Background and purpose

Ca²⁺ -activated Cl^- channels (CaCCs) are gated open by a rise in intracellular Ca²⁺ concentration ([Ca²⁺ ]_i ), typically provoked by activation of G_q -protein coupled receptors (G_q PCR). G_q PCR activation initiates depletion of plasmalemmal phosphatidylinositol 4,5-bisphosphate (PIP₂ ). Here, we determined whether PIP₂ acts as a signalling lipid for CaCCs coded by the TMEM16A and TMEM16B genes.

Experimental approach

Patch-clamp electrophysiology, in conjunction with genetically encoded systems to control cellular PIP₂ content, was used to define the mechanism of action of PIP₂ on TMEM16A and TMEM16B channels.

Key result

SUBMITTER: Ta CM 

PROVIDER: S-EPMC5573538 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature