Analytical methodologies for broad metabolite coverage of exhaled breath condensate.

ABSTRACT: Breath analysis has been gaining popularity as a non-invasive technique that is amenable to a broad range of medical uses. One of the persistent problems hampering the wide application of the breath analysis method is measurement variability of metabolite abundances stemming from differences in both sampling and analysis methodologies used in various studies. Mass spectrometry has been a method of choice for comprehensive metabolomic analysis. For the first time in the present study, we juxtapose the most commonly employed mass spectrometry-based analysis methodologies and directly compare the resultant coverages of detected compounds in exhaled breath condensate in order to guide methodology choices for exhaled breath condensate analysis studies. Four methods were explored to broaden the

SUBMITTER: Aksenov AA 

PROVIDER: S-EPMC5573623 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature