Mi2, an auto-antigen for dermatomyositis, is an ATP-dependent nucleosome remodeling factor.

ABSTRACT: Dynamic changes in chromatin structure play an important role in transcription regulation. Recent studies have revealed two mechanisms that alter chromatin structure. One involves ATP-dependent chromatin remodeling, and the other involves acetylation of the core histone tails. We have previously purified and characterized a multi-subunit protein complex, NuRD, which possesses both nucleosome remodeling and histone deacetylase activities. Despite extensive biochemical characterization of the complex, little is known about the functions of its individual components. In this study, we focused on Mi2, a component of the NuRD complex. We found that, similar to the native NuRD complex, recombinant Mi2 is a DNA-dependent, nucleosome-stimulated ATPase. Kinetic analysis of the ATP hydrolysis reacti

SUBMITTER: Wang HB 

PROVIDER: S-EPMC55739 | biostudies-literature | 2001 Jun

REPOSITORIES: biostudies-literature