Thermo-responsive cell culture carrier: Effects on macrophage functionality and detachment efficiency.

ABSTRACT: Harvesting cultivated macrophages for tissue engineering purposes by enzymatic digestion of cell adhesion molecules can potentially result in unintended activation, altered function, or behavior of these cells. Thermo-responsive polymer is a promising tool that allows for gentle macrophage detachment without artificial activation prior to subculture within engineered tissue constructs. We therefore characterized different species of thermo-responsive polymers for their suitability as cell substrate and to mediate gentle macrophage detachment by temperature shift. Primary human monocyte- and THP-1-derived macrophages were cultured on thermo-responsive polymers and characterized for phagocytosis and cytokine secretion in response to lipopolysaccharide stimulation. We found that both cell typ

SUBMITTER: Rennert K 

PROVIDER: S-EPMC5574476 | biostudies-literature | 2017 Jan-Dec

REPOSITORIES: biostudies-literature