Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples.

ABSTRACT: Molecular techniques have become an important tool to empirically assess feeding interactions. The increased usage of next-generation sequencing approaches has stressed the need of fast DNA extraction that does not compromise DNA quality. Dietary samples here pose a particular challenge, as these demand high-quality DNA extraction procedures for obtaining the minute quantities of short-fragmented food DNA. Automatic high-throughput procedures significantly decrease time and costs and allow for standardization of extracting total DNA. However, these approaches have not yet been evaluated for dietary samples. We tested the efficiency of an automatic DNA extraction platform and a traditional CTAB protocol, employing a variety of dietary samples including invertebrate whole-body extracts as we

SUBMITTER: Wallinger C 

PROVIDER: S-EPMC5574753 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature